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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): For effective gene cloning, a vector should preferably have a single recognition site for a commonly used restriction enzyme. Reason (R): The presence of more than one recognition site for the same enzyme within the vector will generate several fragments, which complicates the gene cloning process.

Options

  1. ABoth (A) and (R) are correct but (R) is not the correct explanation of (A).
  2. B(A) is correct but (R) is not correct.
  3. CBoth (A) and (R) are correct and (R) is the correct explanation of (A).
  4. D(A) is not correct but (R) is correct.

Correct answer

C. Both (A) and (R) are correct and (R) is the correct explanation of (A).

Step-by-step solution

According to NCERT, in order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition site within the vector will generate several fragments, which will complicate the gene cloning process. Therefore, both Assertion (A) and Reason (R) are correct, and (R) is the correct explanation of (A). Answer: Both (A) and (R) are correct and (R) is the correct explanation of (A).

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