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Match List I with List II regarding the separation and isolation of DNA fragments. List I List II A. Agarose I. Extraction of separated DNA bands from the gel piece B. Ethidium bromide II. Required to visualize stained DNA as bright orange bands C. UV radiation III. Natural polymer providing a sieving effect D. Elution IV. Specific dye used for staining DNA fragments Choose the correct answer from the options given b

Options

  1. AA-IV, B-III, C-I, D-II
  2. BA-III, B-IV, C-I, D-II
  3. CA-IV, B-III, C-II, D-I
  4. DA-III, B-IV, C-II, D-I

Correct answer

D. A-III, B-IV, C-II, D-I

Step-by-step solution

Agarose is a natural polymer extracted from seaweeds that provides the sieving effect during gel electrophoresis. Ethidium bromide is a specific compound (dye) used to stain the separated DNA fragments. UV radiation is required to visualize the ethidium bromide-stained DNA, which appears as bright orange bands. Elution is the final step where the separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. Therefore, the correct matching is A-III, B-IV, C-II, D-I. Answer: A-III, B-IV, C

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