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Read the following statements regarding the Polymerase Chain Reaction (PCR): A. Primers used in PCR are small chemically synthesized oligonucleotides complementary to regions of DNA. B. The extension step requires Taq polymerase, which is isolated from a fungus. C. If the process of replication is repeated 30 times, the DNA segment is amplified approximately 1 billion times. D. Denaturation is the final step of a PCR

Options

  1. AOne
  2. BTwo
  3. CThree
  4. DFour

Correct answer

B. Two

Step-by-step solution

Statement A is correct. Primers are small, chemically synthesized oligonucleotides that are complementary to the regions of DNA. Statement B is incorrect. Taq polymerase is a thermostable DNA polymerase isolated from a bacterium, Thermus aquaticus, not a fungus. Statement C is correct. According to NCERT, if the process of replication is repeated 30 times, the DNA segment is amplified approximately 1 billion times. Statement D is incorrect. Denaturation is the first step of each PCR cycle, not the final step. The f

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