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Match List I with List II regarding selectable markers and insertional inactivation. List I List II A. Ligation of alien DNA at Pvu I site in pBR322 1. Susceptibility to Ampicillin B. Ligation of alien DNA at Sal I site in pBR322 2. Susceptibility to Tetracycline C. Intact -galactosidase gene in a plasmid 3. Blue colonies on chromogenic substrate D. Insertional inactivation of -galactosidase gene 4. White colonies on

Options

  1. AA - 2, B - 1, C - 3, D - 4
  2. BA - 1, B - 2, C - 4, D - 3
  3. CA - 1, B - 2, C - 3, D - 4
  4. DA - 2, B - 1, C - 4, D - 3

Correct answer

C. A - 1, B - 2, C - 3, D - 4

Step-by-step solution

In the cloning vector pBR322, the Pvu I restriction site is located within the ampicillin resistance gene. Ligation of alien DNA at this site causes insertional inactivation of this gene, resulting in susceptibility to ampicillin (A-1). The Sal I site is located within the tetracycline resistance gene. Ligation here causes insertional inactivation of this gene, resulting in susceptibility to tetracycline (B-2). When a plasmid contains an intact -galactosidase gene, the bacteria produce the functional enzyme which c

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