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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): In recombinant DNA technology, DNA ligase is required to create hydrogen bonds between the complementary sticky ends of the vector and foreign DNA. Reason (R): Restriction endonucleases inspect the length of a DNA sequence to find their specific recognition sequence and cut the sugar-phosphate back

Options

  1. ABoth Assertion and Reason are true and Reason is the correct explanation of Assertion.
  2. BAssertion is false but Reason is true.
  3. CBoth Assertion and Reason are true but Reason is not the correct explanation of Assertion.
  4. DAssertion is true but Reason is false.

Correct answer

B. Assertion is false but Reason is true.

Step-by-step solution

The Assertion is false. In recombinant DNA technology, the sticky ends of the vector and foreign DNA naturally form hydrogen bonds with their complementary cut counterparts. DNA ligase is not required for hydrogen bonding; rather, it is required to form covalent phosphodiester bonds to seal the sugar-phosphate backbones of the joined DNA fragments. The Reason is true. According to NCERT, each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequen

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