Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEETZoologyBiotechnology - Principles and Processes

A genetic engineer wants to recover a very high number of copies (e.g., 1000 copies per cell) of a target alien DNA. To achieve this, the alien DNA must be cloned into a suitable vector. Which specific sequence of the vector controls this high yield, and which gene codes for the proteins involved in the replication of this plasmid?

Options

  1. AThe 'rop' gene controls the copy number, and the 'ori' sequence codes for the replication proteins.
  2. BThe 'ori' sequence controls the copy number, and the 'rop' gene codes for the replication proteins.
  3. CThe selectable marker controls the copy number, and the 'rop' gene codes for the replication proteins.
  4. DThe 'ori' sequence controls the copy number, and the amp^ R gene codes for the replication proteins.

Correct answer

B. The 'ori' sequence controls the copy number, and the 'rop' gene codes for the replication proteins.

Step-by-step solution

According to NCERT, the origin of replication ('ori') is a sequence from where replication starts. This sequence is also responsible for controlling the copy number of the linked DNA. Therefore, to recover many copies of the target DNA, it should be cloned in a vector whose origin supports a high copy number. The 'rop' gene codes for the proteins involved in the replication of the plasmid. Thus, the 'ori' sequence controls the copy number, and the 'rop' gene codes for the replication proteins. Answer: The 'ori' seq

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs