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Read the following statements regarding the tools of recombinant DNA technology and select the incorrect statement.

Options

  1. ATaq polymerase isolated from Thermus aquaticus remains active during the high temperature induced denaturation
  2. BThe Ti plasmid of Agrobacterium tumefaciens is modified into a non-pathogenic cloning vector to deliver genes
  3. CThe restriction endonuclease EcoRI is isolated from the bacterium Escherichia coli RY 13.
  4. DpBR322 is a naturally occurring plasmid vector isolated directly from Escherichia coli.

Correct answer

D. pBR322 is a naturally occurring plasmid vector isolated directly from Escherichia coli.

Step-by-step solution

pBR322 is not a naturally occurring plasmid; it is an artificially constructed plasmid vector widely used in Escherichia coli. It was created by Bolivar and Rodriguez. Taq polymerase is a thermostable DNA polymerase isolated from the thermophilic bacterium Thermus aquaticus, and it remains active during the high-temperature denaturation step of PCR. The Ti (tumor-inducing) plasmid of Agrobacterium tumefaciens is naturally pathogenic but is disarmed (modified) into a non-pathogenic cloning vector to deliver genes in

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