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Which of the following correctly represents the specific palindromic recognition sequence for the restriction endonuclease EcoRI and its exact sites of cleavage (indicated by arrows)?

Options

  1. A5' - G A A T T C - 3' 3' - C T T A A G - 5'
  2. B5' - G A A T T C - 3' 3' - G A A T T C - 5'
  3. C5' - G A A T T C - 3' 3' - C T T A A G - 5'
  4. D5' - G A A T T C - 3' 3' - C T T A A G - 5'

Correct answer

C. 5' - G A A T T C - 3' 3' - C T T A A G - 5'

Step-by-step solution

Restriction endonuclease EcoRI recognizes the specific palindromic nucleotide sequence 5' - GAATTC - 3' (and 3' - CTTAAG - 5' on the opposite strand). Restriction enzymes cut the DNA helix a little away from the centre of the palindromic sites, but between the same two bases on the opposite strands. EcoRI specifically cuts the phosphodiester bond between the guanine (G) and adenine (A) nucleotides on both strands. Therefore, the correct cleavage pattern is: 5' - G A A T T C - 3' 3' - C T T A A G - 5' Option 1 shows

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