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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): During the isolation of genetic material, the addition of chilled ethanol digests all the remaining proteins and RNA present in the mixture. Reason (R): Purified DNA ultimately precipitates out as fine threads in the suspension after the addition of chilled ethanol. In the light of the above statem

Options

  1. ABoth (A) and (R) are true and (R) is the correct explanation of (A)
  2. BBoth (A) and (R) are true but (R) is not the correct explanation of (A)
  3. C(A) is true but (R) is false
  4. D(A) is false but (R) is true

Correct answer

D. (A) is false but (R) is true

Step-by-step solution

The Assertion is false because chilled ethanol does not digest proteins or RNA. Proteins and RNA are removed earlier in the isolation process by treatment with specific enzymes, namely protease and ribonuclease, respectively. The Reason is true as the final step in the isolation of genetic material involves the addition of chilled ethanol to the purified mixture. This causes the purified DNA to precipitate out, which can be seen as a collection of fine threads in the suspension. Answer: (A) is false but (R) is true

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