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Which of the following double-stranded DNA sequences represents a typical palindromic recognition site for a restriction endonuclease?

Options

  1. A5' - GATTAG - 3' 3' - CTAATC - 5'
  2. B5' - GAATTC - 3' 3' - GAATTC - 5'
  3. C5' - GGATCC - 3' 3' - CCTAGG - 5'
  4. D5' - GTCGTC - 3' 3' - CAGCAG - 5'

Correct answer

C. 5' - GGATCC - 3' 3' - CCTAGG - 5'

Step-by-step solution

A DNA palindrome is a sequence of base pairs that reads the same on the two strands when the orientation of reading is kept the same. For example, if we read 5' 3' on the top strand, the bottom strand must also read the exact same sequence in the 5' 3' direction. In the sequence 5' - GGATCC - 3' and 3' - CCTAGG - 5' , reading the top strand from 5' 3' gives GGATCC. Reading the bottom strand from 5' 3' also gives GGATCC. Furthermore, the two strands correctly follow the rules of complementary base pairing (G pairs w

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