NEETZoologyBiotechnology - Principles and Processes
Match List-I with List-II regarding the components of a cloning vector and their specific functions. List-I List-II A. ori I. Helps in identifying and eliminating non-transformants B. Selectable marker II. Codes for the proteins involved in the replication of the plasmid C. Cloning site III. Initiates replication and controls the copy number of the linked DNA D. rop IV. Recognition site for restriction enzymes to lin
Options
- AA-III, B-I, C-IV, D-II
- BA-II, B-I, C-IV, D-III
- CA-III, B-IV, C-I, D-II
- DA-III, B-II, C-I, D-IV
Correct answer
A. A-III, B-I, C-IV, D-II
Step-by-step solution
According to NCERT, the components of a cloning vector have specific functions: - 'ori' (origin of replication) is a sequence from where replication starts, and it is also responsible for controlling the copy number of the linked DNA (A matches III). - Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants (B matches I). - Cloning sites are the recognition sites for commonly used restriction enzymes where the alien DNA is ligated (C matche