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A researcher is performing a bacterial transformation experiment using a modified plasmid vector. This specific plasmid possesses a functional 'ori' and multiple cloning sites, but it completely lacks any antibiotic resistance genes. What will be the immediate practical limitation of using this vector in the experiment?

Options

  1. AThe plasmid will fail to replicate inside the host cell.
  2. BIt will be impossible to select the transformants from the non-transformants.
  3. CThe foreign DNA cannot be ligated into the vector.
  4. DThe bacterial host cell will die immediately upon transformation.

Correct answer

B. It will be impossible to select the transformants from the non-transformants.

Step-by-step solution

In recombinant DNA technology, antibiotic resistance genes present on a plasmid act as selectable markers. They allow for the selection of transformants (cells that have taken up the plasmid) by permitting their growth on a selective medium containing the antibiotic, while non-transformants are eliminated. Since the modified plasmid lacks antibiotic resistance genes, it cannot provide a selectable marker. Consequently, both transformed and non-transformed cells will grow equally well on a normal medium, making it i

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