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NEETZoologyBiotechnology - Principles and Processes

Read the following statements regarding the separation and isolation of DNA fragments using gel electrophoresis. Choose the incorrect statement.

Options

  1. AThe separated DNA fragments can be visualized directly by exposing the agarose gel to UV radiation.
  2. BSince DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards th
  3. CThe process of cutting out the separated bands of DNA from the agarose gel and extracting them from the gel pi
  4. DDNA fragments stained with ethidium bromide appear as bright orange coloured bands when exposed to UV light.

Correct answer

A. The separated DNA fragments can be visualized directly by exposing the agarose gel to UV radiation.

Step-by-step solution

Option 1 is the incorrect statement because pure DNA fragments cannot be seen directly under visible light or UV radiation without staining. They must first be stained with a specific dye like ethidium bromide before exposure to UV radiation. Option 2 is a correct statement. DNA is negatively charged and moves towards the positive electrode (anode) during electrophoresis. Option 3 is a correct statement. The extraction of the cut DNA bands from the agarose gel piece is termed elution. Option 4 is a correct statemen

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