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During the isolation of genetic material, the purified macromolecules ultimately precipitate out and can be seen as a collection of fine threads in the suspension, which can be removed by spooling. Which of the following treatments specifically causes this precipitation?

Options

  1. AAddition of chilled methanol
  2. BAddition of chilled ethanol
  3. CTreatment with ribonuclease
  4. DAddition of hot ethanol

Correct answer

B. Addition of chilled ethanol

Step-by-step solution

According to NCERT, during the isolation of genetic material, after treating the cellular extract with enzymes like ribonuclease and protease to remove RNA and proteins respectively, the purified DNA ultimately precipitates out after the addition of chilled ethanol. This precipitated DNA is visible as a collection of fine threads in the suspension and can be removed by spooling. Chilled methanol or hot ethanol are not used for this purpose, and ribonuclease is an enzyme used earlier in the process to degrade RNA. A

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