Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEETZoologyBiotechnology - Principles and Processes

Match List-I with List-II regarding the agents used in the isolation of genetic material: List-I List-II (A) Lysozyme (I) Precipitates pure DNA (B) Protease (II) Breaks bacterial cell wall (C) Ribonuclease (III) Degrades RNA (D) Chilled ethanol (IV) Degrades histones Choose the correct answer from the options given below:

Options

  1. A(A)-(II), (B)-(III), (C)-(IV), (D)-(I)
  2. B(A)-(II), (B)-(IV), (C)-(I), (D)-(III)
  3. C(A)-(I), (B)-(IV), (C)-(III), (D)-(II)
  4. D(A)-(II), (B)-(IV), (C)-(III), (D)-(I)

Correct answer

D. (A)-(II), (B)-(IV), (C)-(III), (D)-(I)

Step-by-step solution

During the isolation of genetic material in recombinant DNA technology, different enzymes and chemicals have specific roles. Lysozyme is used to break open bacterial cells to release their intracellular contents. Protease is used to degrade proteins, such as histones, that are intertwined with the DNA. Ribonuclease (RNase) specifically degrades RNA molecules present in the mixture. Chilled ethanol is added at the end of the purification process to precipitate out the purified DNA as fine threads. Therefore, the cor

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs