NEETZoologyBiotechnology - Principles and Processes
Match List-I with List-II. List-I List-II A. Restriction endonuclease I. Joins sugar-phosphate backbones B. DNA ligase II. Reads the same on two strands in 5' 3' direction C. Palindromic sequence III. Inspects the length of a DNA sequence D. Sticky ends IV. Single-stranded overhanging stretches Choose the correct answer from the options given below:
Options
- AA-I, B-III, C-II, D-IV
- BA-III, B-I, C-IV, D-II
- CA-I, B-III, C-IV, D-II
- DA-III, B-I, C-II, D-IV
Correct answer
D. A-III, B-I, C-II, D-IV
Step-by-step solution
Restriction endonucleases function by inspecting the length of a DNA sequence to find their specific recognition site (A-III). DNA ligase is an enzyme that facilitates the joining of DNA fragments by catalyzing the formation of phosphodiester bonds, thus joining the sugar-phosphate backbones (B-I). A palindromic sequence in DNA is a sequence of base pairs that reads the same on the two strands when the orientation of reading is kept the same, such as in the 5' 3' direction (C-II). Sticky ends are single-stranded ov