NEETZoologyBiotechnology - Principles and Processes
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): In a Polymerase Chain Reaction (PCR), there is no need to add fresh DNA polymerase after every cycle. Reason (R): The DNA polymerase isolated from the bacterium Thermus aquaticus remains active during the high temperature induced denaturation of double-stranded DNA. In the light of the above statem
Options
- ABoth (A) and (R) are correct and (R) is the correct explanation of (A).
- BBoth (A) and (R) are correct but (R) is not the correct explanation of (A).
- C(A) is correct but (R) is not correct.
- D(A) is not correct but (R) is correct.
Correct answer
A. Both (A) and (R) are correct and (R) is the correct explanation of (A).
Step-by-step solution
In a Polymerase Chain Reaction (PCR), the process of replication of DNA is repeated many times. The repeated amplification is achieved by the use of a thermostable DNA polymerase (isolated from a bacterium, Thermus aquaticus), which remains active during the high temperature induced denaturation of double stranded DNA. Because this enzyme does not get denatured at high temperatures, it can be added just once at the beginning of the reaction and does not need to be replenished after every cycle. Therefore, both Asse