A student is performing an experiment to isolate pure DNA from a fungal tissue. After successfully removing macromolecules like RNA and proteins from the cellular extract, the student proceeds to the final step to precipitate the purified DNA. Identify the specific enzyme used for the initial lysis of the fungal cells and the chemical added in the final step to precipitate the DNA, respectively.

Step-by-step solution

To isolate DNA from fungal tissue, the fungal cell wall must first be broken down using the enzyme chitinase. Cellulase is used for plant cells and lysozyme for bacterial cells. After the removal of other macromolecules such as RNA (using ribonuclease) and proteins (using protease), the purified DNA is finally precipitated out of the solution by the addition of chilled ethanol. The addition of room-temperature ethanol or methanol is incorrect as chilled ethanol is specifically required for efficient precipitation o