NEETZoologyBiotechnology - Principles and Processes
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): In recombinant DNA technology, it is essential to subject the restriction digest to agarose gel electrophoresis before performing PCR amplification of the gene of interest. Reason (R): Restriction endonucleases cleave the source DNA into multiple fragments, and gel electrophoresis is required to is
Options
- ABoth (A) and (R) are true and (R) is the correct explanation of (A)
- BBoth (A) and (R) are true but (R) is not the correct explanation of (A)
- C(A) is true but (R) is false
- D(A) is false but (R) is true
Correct answer
A. Both (A) and (R) are true and (R) is the correct explanation of (A)
Step-by-step solution
In recombinant DNA technology, the isolation of the desired DNA fragment is a crucial step that follows the cutting of DNA by restriction endonucleases. Restriction enzymes digest the source DNA into a mixture of multiple fragments. To amplify the specific gene of interest using PCR, this desired fragment must first be separated from the rest of the genomic DNA fragments. This separation is achieved using agarose gel electrophoresis, where fragments are separated based on their size. The desired band is then cut ou