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Match the steps of Polymerase Chain Reaction (PCR) in Column I with their corresponding events in Column II. Column I Column II (A) Denaturation (i) Addition of nucleotides to the primer using genomic DNA as template (B) Annealing (ii) Separation of two strands of DNA by heating (C) Extension (iii) Binding of two sets of primers to the complementary regions of DNA Choose the correct option from the following:

Options

  1. A(A)-(iii), (B)-(ii), (C)-(i)
  2. B(A)-(ii), (B)-(i), (C)-(iii)
  3. C(A)-(ii), (B)-(iii), (C)-(i)
  4. D(A)-(i), (B)-(iii), (C)-(ii)

Correct answer

C. (A)-(ii), (B)-(iii), (C)-(i)

Step-by-step solution

Each cycle of Polymerase Chain Reaction (PCR) involves three main steps. Denaturation is the first step, where the double-stranded DNA template is heated to a high temperature to separate the two strands. Annealing is the second step, where two sets of small chemically synthesised oligonucleotides called primers bind to the complementary regions of the separated DNA strands. Extension is the final step, where a thermostable DNA polymerase adds deoxynucleotides to the primers, using the genomic DNA as a template to

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