Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEETZoologyBiotechnology - Principles and Processes

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): In the Polymerase Chain Reaction (PCR), Taq polymerase catalyzes the extension of primers and survives the high-temperature denaturation step. Reason (R): Taq polymerase, isolated from the bacterium Thermus aquaticus, is responsible for breaking the hydrogen bonds between the two strands of DNA at

Options

  1. AAssertion (A) is true but Reason (R) is false.
  2. BBoth Assertion (A) and Reason (R) are true and Reason (R) is the correct explanation of Assertion (A).
  3. CBoth Assertion (A) and Reason (R) are true but Reason (R) is not the correct explanation of Assertion (A).
  4. DAssertion (A) is false but Reason (R) is true.

Correct answer

A. Assertion (A) is true but Reason (R) is false.

Step-by-step solution

Assertion (A) is true because Taq polymerase is a thermostable DNA polymerase that remains active during the high-temperature denaturation step of PCR and catalyzes the extension of primers. Reason (R) is false because the breaking of hydrogen bonds between the two strands of DNA (denaturation) is caused by the application of high heat itself, not by the enzymatic action of Taq polymerase. Taq polymerase only synthesizes the new DNA strand during the extension step and does not act as a helicase to unwind or denatu

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs