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Read the following statements regarding the features of a cloning vector: (A) The 'ori' sequence is responsible for controlling the copy number of the linked DNA. (B) Normal E. coli cells naturally carry resistance genes against antibiotics such as ampicillin and tetracycline. (C) A vector needs to have multiple recognition sites for a single restriction enzyme to facilitate easy gene cloning. (D) Selectable markers

Options

  1. AOnly two
  2. BOnly three
  3. COnly one
  4. DAll four

Correct answer

A. Only two

Step-by-step solution

According to NCERT, the 'ori' (origin of replication) sequence is responsible for controlling the copy number of the linked DNA. Thus, statement A is correct. Normal E. coli cells do not carry resistance against antibiotics like ampicillin, tetracycline, kanamycin, or chloramphenicol. Thus, statement B is incorrect. A cloning vector should preferably have a single recognition site for a commonly used restriction enzyme. The presence of multiple recognition sites generates several fragments, which complicates gene c

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