NEETZoologyBiotechnology - Principles and Processes
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): The Ti plasmid of Agrobacterium tumefaciens must be disarmed before it can be effectively used as a cloning vector. Reason (R): The wild-type Ti plasmid delivers T-DNA that transforms normal plant cells into tumorous cells, which would be detrimental to the host plant. In the light of the above sta
Options
- ABoth (A) and (R) are true but (R) is not the correct explanation of (A).
- B(A) is true but (R) is false.
- CBoth (A) and (R) are true and (R) is the correct explanation of (A).
- D(A) is false but (R) is true.
Correct answer
C. Both (A) and (R) are true and (R) is the correct explanation of (A).
Step-by-step solution
Assertion (A) is true because for a pathogen to be used as a vector to deliver a gene of interest, its disease-causing genes must be removed (disarmed) so it does not harm the host. Reason (R) is true because Agrobacterium tumefaciens is a natural pathogen of several dicot plants. Its wild-type Ti (tumor-inducing) plasmid contains T-DNA that naturally integrates into the plant genome and transforms normal cells into tumorous cells (crown gall). Reason (R) correctly explains Assertion (A) because the very reason the