NEETZoologyBiotechnology - Principles and Processes
A researcher successfully separates DNA fragments using agarose gel electrophoresis. After staining the gel with ethidium bromide and exposing it to UV radiation, characteristic bright orange bands are observed. The researcher then cuts out the desired DNA band from the agarose gel and extracts the DNA from the gel piece. This specific step of cutting and extracting is known as:
Options
- ASpooling
- BElution
- CTransformation
- DDownstream processing
Correct answer
B. Elution
Step-by-step solution
The separation of DNA fragments is carried out by gel electrophoresis. The separated DNA fragments can be visualized only after staining the DNA with ethidium bromide followed by exposure to UV radiation. According to NCERT, the separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This specific step is known as elution. Spooling is the extraction of precipitated DNA from a chilled ethanol suspension during the isolation of genetic material. Transformation is the introduction of