NEETZoologyBiotechnology - Principles and Processes
Which of the following statements regarding the isolation of genetic material in recombinant DNA technology is incorrect?
Options
- ABacterial cells are treated with the enzyme lysozyme to degrade their cell walls and release DNA.
- BFungal cells require treatment with chitinase to break open their cell walls for the release of intracellular
- CAnimal cells must be treated with cellulase and pectinase to dissolve their complex cell walls prior to DNA is
- DPlant cells are treated with cellulase to degrade their cellulosic cell walls to facilitate the release of gen
Correct answer
C. Animal cells must be treated with cellulase and pectinase to dissolve their complex cell walls prior to DNA is
Step-by-step solution
To isolate DNA, the cell must be broken open to release genetic material along with other macromolecules. This is achieved by treating the cells with specific enzymes that degrade their respective cell walls. Bacterial cells are treated with lysozyme, plant cells with cellulase, and fungal cells with chitinase. However, animal cells lack a cell wall entirely; they are bounded only by a plasma membrane. Therefore, they do not require treatment with cell-wall degrading enzymes like cellulase or pectinase. The stateme