Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEETZoologyBiotechnology - Principles and Processes

Match the steps of Polymerase Chain Reaction (PCR) given in List-I with their corresponding key events in List-II. List-I List-II A. Denaturation I. Binding of primers to complementary sequences B. Annealing II. Separation of double-stranded DNA C. Extension III. Addition of nucleotides to the primer Choose the correct answer from the options given below:

Options

  1. AA-II, B-III, C-I
  2. BA-I, B-II, C-III
  3. CA-II, B-I, C-III
  4. DA-III, B-I, C-II

Correct answer

C. A-II, B-I, C-III

Step-by-step solution

Polymerase Chain Reaction (PCR) completes one cycle in three steps: 1. Denaturation: The double-stranded template DNA is heated to a high temperature, causing the two strands to separate. 2. Annealing: The temperature is lowered to allow short oligonucleotide primers to bind to their complementary sequences on the single-stranded DNA. 3. Extension: Taq DNA polymerase adds deoxynucleotides to the primers, extending the new DNA strand. Therefore, the correct matching is A-II, B-I, C-III. Answer: A-II, B-I, C-III

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs