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Match List I with List II regarding the restriction sites in the cloning vector pBR322. List I (Restriction enzyme) List II (Location / Consequence of insertion) (a) Pvu I (i) Inactivates tet^R gene (b) BamH I (ii) Located in rop gene (c) Pvu II (iii) Located outside both resistance genes (d) Hind III (iv) Inactivates amp^R gene Choose the correct answer from the options given below:

Options

  1. A(a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
  2. B(a)-(i), (b)-(iv), (c)-(ii), (d)-(iii)
  3. C(a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
  4. D(a)-(iv), (b)-(i), (c)-(ii), (d)-(iii)

Correct answer

D. (a)-(iv), (b)-(i), (c)-(ii), (d)-(iii)

Step-by-step solution

In the cloning vector pBR322: - The restriction site for Pvu I is located within the ampicillin resistance ( amp^R ) gene. Insertion of foreign DNA here inactivates the amp^R gene. - The restriction site for BamH I is located within the tetracycline resistance ( tet^R ) gene. Insertion here inactivates the tet^R gene. - The restriction site for Pvu II is located in the rop gene, which codes for the proteins involved in the replication of the plasmid. - The restriction site for Hind III is located outside both the a

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