Read the following statements regarding the principles and processes of recombinant DNA technology: A. DNA fragments are negatively charged molecules and they move towards the anode under an electric field in agarose gel electrophoresis. B. Polymerase chain reaction (PCR) requires a thermostable DNA polymerase isolated from a bacterium. C. In recombinant DNA technology, the ligation of the desired gene into a vector

Step-by-step solution

Statement A is correct: DNA fragments are negatively charged and move towards the positive electrode (anode) during gel electrophoresis. Statement B is correct: PCR uses Taq polymerase, a thermostable DNA polymerase isolated from the bacterium Thermus aquaticus. Statement C is incorrect: In recombinant DNA technology, the gene of interest is typically amplified using PCR first, and then this amplified fragment is ligated with a vector for further cloning. Statement D is correct: During the isolation of genetic mate