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A researcher isolates a mutant form of the E. coli cloning vector pBR322 that has suffered a complete deletion of a specific gene. Consequently, the plasmid is unable to produce the proteins involved in its own replication. Which of the following restriction endonucleases will fail to cleave this mutant plasmid due to the loss of its recognition site?

Options

  1. APvu II
  2. BPvu I
  3. CPst I
  4. DBamH I

Correct answer

A. Pvu II

Step-by-step solution

The gene responsible for coding the proteins involved in the replication of the plasmid pBR322 is the 'rop' gene. According to the map of pBR322, the restriction site for the enzyme Pvu II is located within the 'rop' gene. If the 'rop' gene is completely deleted, its corresponding restriction site is also lost. Therefore, Pvu II will no longer be able to cleave this mutant plasmid. Pvu I and Pst I sites are located in the ampicillin resistance gene ( amp^ R ), and the BamH I site is located in the tetracycline resi

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