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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Recombinant E. coli produced after inserting foreign DNA at the Pst I site of pBR322 will grow on a medium containing tetracycline but not on a medium containing ampicillin. Reason (R): The Pst I restriction site is located within the ampicillin resistance gene, and insertion of foreign DNA here le

Options

  1. ABoth Assertion and Reason are true and Reason is the correct explanation of Assertion.
  2. BBoth Assertion and Reason are true but Reason is not the correct explanation of Assertion.
  3. CAssertion is true but Reason is false.
  4. DAssertion is false but Reason is true.

Correct answer

A. Both Assertion and Reason are true and Reason is the correct explanation of Assertion.

Step-by-step solution

In the cloning vector pBR322, the restriction sites Pst I and Pvu I are located within the ampicillin resistance gene ( amp^ R ). When foreign DNA is inserted at the Pst I site, the amp^ R gene undergoes insertional inactivation. As a result, the recombinant plasmid loses the ability to confer resistance to ampicillin, while retaining resistance to tetracycline (since the tet^ R gene remains intact). Thus, the recombinant E. coli transformants will grow on a medium containing tetracycline but will fail to grow on a

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