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During an agarose gel electrophoresis experiment, three different double-stranded DNA samples, A, B, and C, were loaded into separate wells. After running the gel for a specific duration, the migration distances of the DNA bands from the wells were measured as follows: Sample A: 4 cm Sample B: 2 cm Sample C: 6 cm Assuming all other conditions are identical, which of the following represents the correct ascending orde

Options

  1. AC < A < B
  2. BB < A < C
  3. CA < B < C
  4. DA < C < B

Correct answer

A. C < A < B

Step-by-step solution

In agarose gel electrophoresis, DNA fragments separate according to their size through the sieving effect provided by the agarose gel. The smaller the fragment size (lower molecular weight), the farther it moves from the well. Therefore, the migration distance of a DNA fragment is inversely proportional to its molecular weight. Given the migration distances: Sample C ( 6 cm ) > Sample A ( 4 cm ) > Sample B ( 2 cm ) The molecular weights will follow the reverse order: Sample C Thus, the correct ascending order of th

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