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Read the following statements regarding restriction enzymes: (A) Hind II was the first restriction endonuclease to be isolated. (B) Hind II always cuts DNA molecules at a particular point by recognising a specific sequence of 8 base pairs. (C) Restriction endonucleases cut the DNA molecule by breaking the hydrogen bonds between the nitrogenous bases. (D) The specific base sequence recognised by a restriction endonucl

Options

  1. AOne
  2. BTwo
  3. CThree
  4. DFour

Correct answer

B. Two

Step-by-step solution

Statement (A) is correct as Hind II was the first restriction endonuclease to be isolated and characterised. Statement (B) is incorrect because Hind II recognises a specific sequence of 6 base pairs, not 8 base pairs. Statement (C) is incorrect because restriction endonucleases cut the sugar-phosphate backbone of the DNA strands by cleaving phosphodiester bonds, not the hydrogen bonds between the nitrogenous bases. Statement (D) is correct as the specific sequence of DNA recognised and cut by the enzyme is called i

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