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A researcher successfully isolated purified DNA from a biological sample by first treating the cells with a specific enzyme mixture containing chitinase, protease, and ribonuclease, followed by precipitation with chilled ethanol. Which of the following organisms was most likely the source of the sample?

Options

  1. AStreptococcus pneumoniae
  2. BSaccharomyces cerevisiae
  3. CMangifera indica
  4. DHomo sapiens

Correct answer

B. Saccharomyces cerevisiae

Step-by-step solution

In recombinant DNA technology, the isolation of genetic material requires the disruption of the cell boundary to release DNA along with other macromolecules. The type of enzyme used depends on the biochemical composition of the cell wall. The use of chitinase indicates that the organism has a cell wall composed of chitin, which is a characteristic feature of fungi. Among the given options, Saccharomyces cerevisiae (yeast) is a fungus. Streptococcus pneumoniae is a bacterium (which would require lysozyme), Mangifera

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