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Match List-I with List-II regarding the features of the cloning vector pBR322. List-I List-II A. ori I. Contains Pvu I site B. rop II. Contains BamH I site C. amp^ R III. Controls copy number of linked DNA D. tet^ R IV. Codes for proteins involved in replication Choose the correct answer from the options given below:

Options

  1. AA-IV, B-III, C-I, D-II
  2. BA-III, B-IV, C-II, D-I
  3. CA-IV, B-III, C-II, D-I
  4. DA-III, B-IV, C-I, D-II

Correct answer

D. A-III, B-IV, C-I, D-II

Step-by-step solution

In the cloning vector pBR322: - 'ori' (origin of replication) is the sequence responsible for initiating replication and controlling the copy number of the linked DNA. - 'rop' codes for the proteins involved in the replication of the plasmid. - The ampicillin resistance gene ( amp^ R ) contains the recognition sites for the restriction endonucleases Pvu I and Pst I. - The tetracycline resistance gene ( tet^ R ) contains the recognition sites for BamH I and Sal I. Therefore, the correct matching is A-III, B-IV, C-I,

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