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In recombinant DNA technology, various methods are employed to introduce alien DNA into host cells. Which of the following is a direct (vectorless) method of gene transfer?

Options

  1. AAgrobacterium-mediated transfer
  2. BRetrovirus infection
  3. CBacteriophage infection
  4. DMicro-injection

Correct answer

D. Micro-injection

Step-by-step solution

Agrobacterium tumefaciens, retroviruses, and bacteriophages act as biological vectors to carry and deliver foreign DNA into specific host cells. Micro-injection is a direct (vectorless) method of gene transfer in which recombinant DNA is directly injected into the nucleus of an animal cell using a micro-pipette, without the involvement of any biological vector. Answer: Micro-injection

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