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Match List-I with List-II regarding the enzymes used during the isolation of genetic material. List-I List-II (A) Plant cells (I) Lysozyme (B) Fungal cells (II) Chitinase (C) RNA (III) Protease (D) Proteins (IV) Ribonuclease (V) Cellulase Choose the correct answer from the options given below:

Options

  1. A(A)-(I), (B)-(II), (C)-(IV), (D)-(III)
  2. B(A)-(V), (B)-(II), (C)-(IV), (D)-(III)
  3. C(A)-(II), (B)-(V), (C)-(IV), (D)-(III)
  4. D(A)-(V), (B)-(II), (C)-(III), (D)-(IV)

Correct answer

B. (A)-(V), (B)-(II), (C)-(IV), (D)-(III)

Step-by-step solution

During the isolation of genetic material, the cells must be broken open to release DNA along with other macromolecules. Plant cells are treated with cellulase to degrade their cellulosic cell wall. Fungal cells are treated with chitinase to break down the chitin in their cell walls. Once the cell is open, RNA is removed by treatment with ribonuclease, whereas proteins are removed by treatment with protease. Lysozyme is used to break open bacterial cells, not plant cells. Swapping the enzymes for RNA and proteins, o

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