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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): During the isolation of pure DNA from a cell, the enzyme DNase is strictly avoided, whereas ribonuclease and protease are added to the cellular lysate. Reason (R): Ribonuclease and protease purify the DNA by degrading RNA and proteins respectively, but DNase would destroy the target genetic materia

Options

  1. ABoth (A) and (R) are true and (R) is the correct explanation of (A)
  2. BBoth (A) and (R) are true but (R) is not the correct explanation of (A)
  3. C(A) is true but (R) is false
  4. D(A) is false but (R) is true

Correct answer

A. Both (A) and (R) are true and (R) is the correct explanation of (A)

Step-by-step solution

For the isolation of pure DNA, contaminating macromolecules such as RNA and proteins must be removed from the cellular extract. This purification is achieved by treating the lysate with ribonuclease (RNase) to degrade RNA and protease to degrade proteins. The enzyme DNase (deoxyribonuclease) specifically degrades DNA. If DNase were added, it would destroy the target genetic material being isolated. Therefore, DNase is strictly avoided. Both the Assertion and Reason are true, and the Reason correctly explains why DN

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