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Match the restriction endonucleases in List I with their specific locations on the pBR322 cloning vector in List II. List I List II A. Pst I I. tet^R gene B. Bam HI II. rop gene C. Pvu II III. amp^R gene D. Eco RI IV. Region outside selectable markers Choose the correct answer from the options given below:

Options

  1. AA-III, B-I, C-II, D-IV
  2. BA-I, B-III, C-II, D-IV
  3. CA-III, B-I, C-IV, D-II
  4. DA-II, B-I, C-III, D-IV

Correct answer

A. A-III, B-I, C-II, D-IV

Step-by-step solution

The pBR322 cloning vector has specific recognition sites for various restriction endonucleases. Pst I has its recognition site within the ampicillin resistance ( amp^R ) gene (A matches III). Bam HI has its recognition site within the tetracycline resistance ( tet^R ) gene (B matches I). Pvu II has its recognition site within the rop gene, which codes for the proteins involved in the replication of the plasmid (C matches II). Eco RI has its recognition site at the top region of the plasmid, outside the selectable m

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