Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEETZoologyBiotechnology - Principles and Processes

Read the following statements regarding the tools used in recombinant DNA technology: I. Exonucleases remove nucleotides from the ends of DNA. II. Endonucleases make cuts at specific positions within the DNA. III. DNA ligase facilitates the joining of DNA fragments by forming hydrogen bonds between them. IV. Taq polymerase is isolated from a thermophilic bacterium and extends primers during PCR. How many of the given

Options

  1. AOne
  2. BTwo
  3. CThree
  4. DFour

Correct answer

C. Three

Step-by-step solution

Statement I is correct as exonucleases remove nucleotides from the free ends of DNA. Statement II is correct as endonucleases make cuts at specific positions within the DNA duplex. Statement III is incorrect because DNA ligase joins DNA fragments by forming covalent phosphodiester bonds in the sugar-phosphate backbone, not hydrogen bonds (hydrogen bonds form spontaneously between complementary sticky ends). Statement IV is correct as Taq polymerase is a thermostable enzyme isolated from the thermophilic bacterium T

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs