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Which of the following representations correctly shows the palindromic sequence and the exact cleavage sites (indicated by arrows) for the restriction endonuclease EcoRI?

Options

  1. A5' G A A T T C 3' 3' C T T A A G 5'
  2. B5' G A A T T C 3' 3' C T T A A G 5'
  3. C5' G A A T T C 3' 3' C T T A A G 5'
  4. D5' G A A T T C 3' 3' C T T A A G 5'

Correct answer

D. 5' G A A T T C 3' 3' C T T A A G 5'

Step-by-step solution

EcoRI recognizes the 6-base pair palindromic sequence 5' - G A A T T C - 3'. It does not cut exactly in the center of the palindrome (which would produce blunt ends). Instead, it cuts between the same two bases on the opposite strands, specifically between the G and A bases. This staggered cut generates overhanging stretches called sticky ends. Therefore, the correct cleavage site is between G and A on the 5' 3' strand, and between A and G on the 3' 5' strand. Answer: 5' G A A T T C 3' 3' C T T A A G 5'

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