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Read the following statements regarding the cloning vector pBR322: (A) It contains two selectable markers, amp^ R and tet^ R . (B) The recognition site for the restriction enzyme BamH I is located within the amp^ R gene. (C) Insertion of alien DNA at the Sal I site results in insertional inactivation of the tetracycline resistance gene. (D) The 'rop' gene codes for the proteins involved in the replication of the plas

Options

  1. ATwo
  2. BThree
  3. CFour
  4. DOne

Correct answer

B. Three

Step-by-step solution

Statement (A) is correct as pBR322 contains ampicillin ( amp^ R ) and tetracycline ( tet^ R ) resistance genes as selectable markers. Statement (B) is incorrect because the recognition site for BamH I is located within the tet^ R gene, not the amp^ R gene. The amp^ R gene contains recognition sites for Pvu I and Pst I. Statement (C) is correct because Sal I has a recognition site within the tet^ R gene. Insertion of alien DNA here disrupts the gene, leading to insertional inactivation of tetracycline resistance. St

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