Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): To construct a recombinant DNA molecule, it is mandatory to digest both the plasmid vector and the alien DNA with the same restriction endonuclease. Reason (R): DNA ligase is the enzyme responsible for linking the sticky ends of the vector and the alien DNA. In the light of the above statements, ch

Step-by-step solution

Assertion (A) is correct because, according to NCERT, unless one cuts the vector and the source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created. Reason (R) is also correct as DNA ligase is indeed the enzyme that joins the DNA fragments by forming phosphodiester bonds between the sticky ends. However, Reason (R) is not the correct explanation for Assertion (A). The correct explanation is that cutting both the vector and the alien DNA with the same restriction enzyme produces f