NEETZoologyBiotechnology - Principles and Processes
Read the following statements regarding the methods of introducing alien DNA into host cells: I. In the biolistics method, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA. II. Micro-injection is a method in which recombinant DNA is directly injected into the nucleus of a plant cell. III. Agrobacterium tumefaciens delivers a piece of DNA known as 'T-DNA' to transform normal a
Options
- AOne
- BThree
- CFour
- DTwo
Correct answer
D. Two
Step-by-step solution
Statement I is correct: Biolistics or gene gun method involves bombarding cells with high velocity micro-particles of gold or tungsten coated with DNA, and is primarily used for plant cells. Statement II is incorrect: Micro-injection is a method used to directly inject recombinant DNA into the nucleus of an animal cell, not a plant cell. Statement III is incorrect: Agrobacterium tumefaciens is a pathogen of several dicot plants, not animals. It delivers T-DNA to transform normal plant cells into a tumor. Statement