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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): In recombinant DNA technology, selection of recombinants using antibiotic resistance genes is preferred over alternative selectable markers like the -galactosidase gene. Reason (R): Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because it requires simultaneo

Options

  1. ABoth Assertion (A) and Reason (R) are true and Reason (R) is the correct explanation of Assertion (A)
  2. BBoth Assertion (A) and Reason (R) are true but Reason (R) is not the correct explanation of Assertion (A)
  3. CAssertion (A) is true but Reason (R) is false
  4. DAssertion (A) is false but Reason (R) is true

Correct answer

D. Assertion (A) is false but Reason (R) is true

Step-by-step solution

Assertion (A) is false because alternative selectable markers (like the -galactosidase gene) are actually preferred over antibiotic resistance genes. They were developed specifically to overcome the difficulties associated with antibiotic-based selection. Reason (R) is a true statement. According to NCERT, the selection of recombinants due to the insertional inactivation of antibiotic resistance genes is a cumbersome procedure. It requires simultaneous plating on two different antibiotic plates to distinguish recom

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