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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): If one wants to recover many copies of the target DNA, it should be cloned in a vector whose origin supports a high copy number. Reason (R): The 'ori' sequence is responsible for controlling the copy number of the linked DNA. In the light of the above statements, choose the most appropriate answer

Options

  1. ABoth Assertion (A) and Reason (R) are correct but Reason (R) is not the correct explanation of Assertion (A).
  2. BAssertion (A) is correct but Reason (R) is not correct.
  3. CBoth Assertion (A) and Reason (R) are correct and Reason (R) is the correct explanation of Assertion (A).
  4. DAssertion (A) is not correct but Reason (R) is correct.

Correct answer

C. Both Assertion (A) and Reason (R) are correct and Reason (R) is the correct explanation of Assertion (A).

Step-by-step solution

Assertion (A) is correct because to achieve a high yield of recombinant DNA, the vector must be capable of supporting multiple copies within the host cell. Reason (R) is also correct because the 'ori' (origin of replication) is the specific sequence that not only initiates replication but also controls the copy number of the linked DNA. Therefore, Reason (R) provides the correct biological explanation for why a specific vector (based on its 'ori') must be chosen to satisfy the goal stated in Assertion (A). Answer:

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