NEETZoologyBiotechnology - Principles and Processes
In a specific vectorless gene transfer method, recombinant DNA is directly introduced into the nucleus of the host cell. Identify the name of this method and the type of host cells it is primarily suitable for.
Options
- AGene gun method, Plant cells
- BMicro-injection, Animal cells
- CMicro-injection, Plant cells
- DHeat shock method, Bacterial cells
Correct answer
B. Micro-injection, Animal cells
Step-by-step solution
According to NCERT, in the micro-injection method, recombinant DNA is directly injected into the nucleus of an animal cell. In contrast, the gene gun (biolistics) method is primarily suitable for plant cells, and the heat shock method (along with divalent cation treatment) is used to make bacterial cells competent to take up DNA. Answer: Micro-injection, Animal cells