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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Eco RI cuts the DNA sequence 5'-GAATTC-3' strictly between the bases G and A on both the strands. Reason (R): Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site, but between the same two bases on the opposite strands. In the light of the above stateme

Options

  1. ABoth (A) and (R) are correct and (R) is the correct explanation of (A)
  2. BBoth (A) and (R) are correct but (R) is not the correct explanation of (A)
  3. C(A) is correct but (R) is incorrect
  4. D(A) is incorrect but (R) is correct

Correct answer

A. Both (A) and (R) are correct and (R) is the correct explanation of (A)

Step-by-step solution

Assertion (A) is correct because Eco RI specifically recognizes the palindromic sequence 5'-GAATTC-3' and cuts the DNA between the G and A bases on both strands. Reason (R) is also correct because restriction enzymes that produce sticky ends (like Eco RI) follow the general mechanism of cutting the DNA strand a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. The Reason correctly explains the Assertion, as the specific cleavage site of Eco RI (between G an

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