NEETZoologyBiotechnology - Principles and Processes
In a method of introducing alien DNA into competent host cells, the cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA. Identify the name of this method and the specific type of host cell it is primarily suitable for.
Options
- ABiolistics, Plant cells
- BMicro-injection, Animal cells
- CBiolistics, Animal cells
- DDisarmed retrovirus, Plant cells
Correct answer
A. Biolistics, Plant cells
Step-by-step solution
The method described is biolistics or the gene gun method, where cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA. This method is primarily suitable for introducing recombinant DNA into plant cells. Micro-injection is a different method where recombinant DNA is directly injected into the nucleus of an animal cell using a micropipette. Disarmed retroviruses are used as vectors to deliver desirable genes into animal cells, not plant cells. Answer: Biolistics, Plant cells