Match List-I with List-II: List-I List-II A. Denaturation I. Binding of oligonucleotide primers B. Annealing II. Thermus aquaticus C. Extension III. Synthesis of new DNA strand D. Taq polymerase IV. Separation of dsDNA at high temperature Choose the correct answer from the options given below:

Step-by-step solution

Denaturation is the first step of PCR where double-stranded DNA is heated to a high temperature to separate the strands (A-IV). Annealing involves the binding of small chemically synthesized oligonucleotide primers to the separated DNA strands (B-I). Extension is the synthesis of a new DNA strand by a DNA polymerase using the primers and deoxynucleotides (C-III). Taq polymerase is a thermostable DNA polymerase isolated from the bacterium Thermus aquaticus (D-II). Therefore, the correct matching is A - IV, B - I, C