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Match List-I with List-II regarding the restriction enzyme sites in the cloning vector pBR322. List-I (Restriction Enzyme) List-II (Location in pBR322) A. PvuI I. amp^ R gene B. BamHI II. tet^ R gene C. PvuII III. rop gene D. EcoRI IV. Outside the antibiotic resistance genes Choose the correct answer from the options given below:

Options

  1. AA-III, B-II, C-I, D-IV
  2. BA-II, B-I, C-III, D-IV
  3. CA-I, B-II, C-III, D-IV
  4. DA-I, B-II, C-IV, D-III

Correct answer

C. A-I, B-II, C-III, D-IV

Step-by-step solution

In the cloning vector pBR322: The restriction site for PvuI is located within the ampicillin resistance gene ( amp^ R ). The restriction site for BamHI is located within the tetracycline resistance gene ( tet^ R ). The restriction site for PvuII is located within the rop gene, which codes for the proteins involved in the replication of the plasmid. The restriction site for EcoRI is located outside the antibiotic resistance genes. Thus, the correct matching is A-I, B-II, C-III, D-IV. Answer: A-I, B-II, C-III, D-IV

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